Data-Driven Visualizations of Embryogenesis

Comparative Anatomy

The term morphology has been coined by Goethe in 1790 in his book Metamorphosis of Plants where he defines the concept of an original ideal plant “Urpflanze” from which all plants are derived by simple transformations.

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Representation of Goethe’s Urpflanze

In 1859, Darwin published On the Origin of Species where he theorized about the relationships between species. The transformations between species occur continuously at each generation through a process of descent with random modification. This understanding of the evolution of species comes from comparative anatomy.

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First drawing of Darwin’s phylogenetic tree

Darwin’s work changed the world’s view on diversity and forms in nature. The observation and comparison of anatomy became a source of knowledge. Haeckel was a german naturalist who published artwork of comparative anatomy in 1904 in the book Kunstformen der Natur.

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Ascidian – Kunstformen der Natur

In 1917, D’Arcy Wentworth Thomson published On Growth And Form. As a mathematician, a physicist and a biologist, he worked on defining general laws to explain morphogenesis and transformation between species. His book has had a major influence during the 20th century.

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Comparison of fish species morphologies using a common grid – On Growth and Form

In 1952, mathematician Alan Turing published The chemical basis of morphogenesis which remains a reference for our understanding of the emergence of patterns in homogeneous tissue. He shows using a non-linear system of equations how random local perturbations can lead to large scale spatial patterns.

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Dappled pattern obtained from a simulation of a reaction diffusion system – Turing, 1952

This is one of the first attempts at explaining morphology as a self-organized system.

Embryogenesis

Embryogenesis is the process by which a single fertilized cell turns into a multicellular organism. Danio rerio, a.k.a. zebrafish, is a vertebrate model organism widely used in scientific research for studies of development.

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Adult Zebrafish

This example shows how the processes of cell proliferation and cell motility combine into complex morphogenetic movements to form a highly structured multicellular organism.

Recent developments in microscopy imaging technologies have enabled the visualization of development at the single cell resolution.

The Drosophila melanogaster, a.k.a. fruit fly, is a major model organism for the study of developmental genetics. Here is an example of light sheet microscopy of the first hours of fruit fly development at single cell resolution.

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Adult fruitfly

In addition to cell proliferation and tissue morphogenesis, cells are differentiating during embryonic development. Even though each cell carry the same DNA, some of them will end up becoming muscles cells, whereas others will become part of the nervous system. Cell differentiation mainly occurs through changes in molecular content, such as protein distribution. The protein spatial localization is highly reproducible during development and lead to beautiful patterns.

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Fruitfly embryo with nuclei and two proteins stained – Lim et al., 2015

Many images of gene expression are shared in openly accessible databases: Berkeley Drosophila Transcription Network Project, and the FlyEx Database.

Understanding the interplay between gene expression, protein spatial distribution and morphogenesis requires keeping track of the various dynamics in a dynamical atlas.

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Atlas of dpERK during embryogenesis – Gabay et al., 1997

This atlas of gene expression has been obtained mainly by hand 20 years ago. One current challenge of developmental biology is to develop method for automatizing the construction of this kind of atlas using data-driven techniques.

In all areas of biology, there is a need for new visualizations to help researchers make sense of new and massive data sets.

Other model organisms are used for the study of cellular process, for example the sea urchin.

Developmental biologist Benny Shilo wrote a book explaining the main concepts of embryology using photographs from everyday life.

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Illustration of the fact that the accumulation of simple decisions during development leads to increased complexity. In the same way, the game of dominoes leads to complex forms – Benny Shilo, 2014

 

Human embryogenesis and Art

Visual representation of human embryonic development began as early as in Leonardo Da Vinci’s drawings in 1510.

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The fetus in the womb

Photographer Lennart Nilson has photographed embryos at various stages of growth, culminating in the bestseller 1965 book A child is born. The reception was very enthusiastic, see for example the cover of Life magazine.

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More recently, embryos have been shown as sculptures in Gunther von Hagen’s Body World exhibit.

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Reclining Pregnant Woman

Morphogenesis in Architecture and Design

The concepts of morphogenesis have been used as sources of inspiration in architecture and design.

Architect Antoni Gaudí has taken inspiration from natural forms and growth patterns to design his numerous architectural realizations.

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Sagrada Familia Nave Roof

Designer and architect Neri Oxman develops computational approaches using 3D printing that are directly inspired by morphogenesis in nature.

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Mushtari – Neri Oxman, 2014

Written by Paul Villoutreix

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